HOG KIDNEY GLUCONOKINASE

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Hog kidney gluconokinase.

Previous studies (1, 2) have indicated that gluconate is extensively metabolized in the mammal without, prior conversion to glucose. The int,erpretation of experiments performed with labeled gluconate has rested upon the assumption (3) that gluconate is converted to 6-phosphogluconate in t,he course of its metabolism. The enzyme, gluconokinase, which catalyzes this reaction has been partially p...

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Studies of hog kidney acylase I. I. Comparison with hog kidney dialkylfluorophosphatase.

Hog kidney Acylase I, an enzyme which hydrolyzes N-acetylL-amino acids, has been purified approximately 40-fold by Greenstein (1). During the course of the purification of hog kidney dialkylfluorophosphatase, it was consistently found that the protein fractions containing DFPasel activity also possessed very high Acylase I activity. Inasmuch as the DFPase and Acylase I activities were found to ...

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Fluorescence properties of hog kidney aminoacylase I.

The state of the tryptophan residues of porcine kidney aminoacylase I (EC 3.5.1.14) was investigated by fluorescence spectroscopy and chemical modification. The pH-dependence of the fluorescence emission spectrum of the enzyme indicates that its native conformation prevails between pH 6 and 9.5. Within this range, the ionization of a residue with an apparent pKa of 7.1 quenches the enzyme fluor...

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Observations on vitamin Bc conjugase from hog kidney.

Some time ago we called attention to the wide-spread occurrence in nature of an enzyme which releases vitamin B, from a microbiologically inactive form (1). In work designed to characterize the enzyme in hog kidney through a study of this reaction it became apparent that the kinetics of the reaction were very markedly influenced by the purity of the substrate. With the isolation of vitamin B, c...

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Observations on Vitamin B, Conjugase from Hog Kidney

Some time ago we called attention to the wide-spread occurrence in nature of an enzyme which releases vitamin B, from a microbiologically inactive form (1). In work designed to characterize the enzyme in hog kidney through a study of this reaction it became apparent that the kinetics of the reaction were very markedly influenced by the purity of the substrate. With the isolation of vitamin B, c...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1957

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)64915-4